Fig. 2: L-PC increases the level of miR-21-3p and miR-21-3p enhances the M1 phenotype in macrophages. | Communications Biology

Fig. 2: L-PC increases the level of miR-21-3p and miR-21-3p enhances the M1 phenotype in macrophages.

From: MiR-21-3p inhibitor exerts myocardial protective effects by altering macrophage polarization state and reducing excessive mitophagy

Fig. 2

A qRT-PCR detection of miR-21-3p levels in serum of patients with CHF. *P < 0.05 vs. normal. Experiment A consisted of 12 biological replicates, each representing the average of 3 technical replicates. B qRT-PCR detection of miR-21-3p levels in serum and heart of rats. C Pearson correlation analysis of miR-21-3p levels and CPT1A levels in heart. D Flow cytometry analysis of the effect of L-PC on the enrichment of macrophage surface markers CD86 and CD206 in spleen tissues of ISO-induced model. *P < 0.05 vs. control. &P < 0.05 vs. ISO. E qRT-PCR detection of miR-21-3p levels in Mφ, M1 and M2 macrophages. *P < 0.05 vs. Mφ. F qRT-PCR detection of the secretion of cytokines IL-1β and TNF-α in M1 macrophages by miR-21-3p. G Flow cytometry analysis of the effect of miR-21-3p on the surface marker CD86 in M1 macrophages. *P < 0.05 vs. M1. Experiments B to G consisted of 6 biological replicates, each representing the average of 3 technical replicates.

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