Fig. 3: miR-21-3p binds to CPT1A and promotes excessive mitophagy in H9c2 cells. | Communications Biology

Fig. 3: miR-21-3p binds to CPT1A and promotes excessive mitophagy in H9c2 cells.

From: MiR-21-3p inhibitor exerts myocardial protective effects by altering macrophage polarization state and reducing excessive mitophagy

Fig. 3

A Prediction of potential binding sites of miR-21-3p to CPT1A using miRmap. B Validation of miR-21-3p binding to CPT1A by dual-LUC assay. *P < 0.05 vs. CPT1A-WT+NC mimics. C qRT-PCR analysis of miR-21-3p levels in H9c2 cells. D Evaluation of the effect of miR-21-3p on cell viability in H9c2 cells using CCK8 assay. E Analysis of the effects of miR-21-3p on the protein levels of LC3 II, LC3I, p62 and CPT1A in H9c2 cells using Western blotting. F Observation of the effect of miR-21-3p on the number of autophagosome-like structures in H9c2 cells by transmission electron microscopy. Scale bars: 2 μm and 500 nm. *P < 0.05 vs. control. & P < 0.05 vs. NC inhibitor. G qRT-PCR analysis of miR-21-3p and CPT1A mRNA levels in H9c2 cells. H Evaluation of the effect of CPT1A and miR-21-3p on cell viability in H9c2 cells using CCK8 assay. I Western blotting analysis of the effects of CPT1A and miR-21-3p on the protein levels of LC3 II, LC3I, p62, and CPT1A in H9c2 cells. J Observation of the effects of CPT1A and miR-21-3p on the number of autophagosome-like structures in H9c2 cells by transmission electron microscopy. Scale bars: 2 μm and 500 nm. K IF staining showing the subcellular localization of miR-21-3p and CPT1A in H9c2 cells. Scale bar: 25 μm. *P < 0.05 vs. oe-NC. &P < 0.05 vs. oe-CPT1A+NC mimic. All experiments consisted of 6 biological replicates, each representing the average of 3 technical replicates.

Back to article page