Fig. 3: Spatial and photon sparsity enhancement by SparseFLIM.
From: Overcoming photon and spatiotemporal sparsity in fluorescence lifetime imaging with SparseFLIM
a Network architecture of SU SparseFLIM. \({{{{\boldsymbol{U}}}}}_{{{{\boldsymbol{s}}}}}\), spatial upsampling module. CP collected pixels; UnCP uncollected pixels. b Input images of fluorescent beads and the corresponding network reconstruction results. Yellow dashed circles indicate invisible beads that are clearly resolved by the network. c Close-up images showing a pair of beads. The solid line in the images refers to the line of the shown cross-section. d Comparison of the input and output images of a skin tissue. The photon-rich image is presented as reference. e Tukey box-and-whisker plot illustrating 3D SNR changes in skin data between bicubic upsampling of the input and SU SparseFLIM result (n = 46 \({{{\boldsymbol{x}}}}\)-\({{{\bf{y}}}}\)-\({{{\boldsymbol{t}}}}\) stacks). f FSC measure on bicubic upsampling of the input and SU SparseFLIM result. Two-tailed Wilcoxon matched-pairs signed rank tests were applied between the input and output in (e). Scale bars, 20 μm in (b, c), 100 μm in (d).
