Fig. 2: Trajectories of zoospore movement observed using phase-contrast microscopy.

Sporangium dehiscence was induced by pouring 25 mM NH4HCO3 solution onto sporangium-forming HAT agar, followed by incubation at room temperature for 1 h. Zoospores released from sporangia were observed. a The PrpoB-ftgA strain. b The S-7 strain. c The fliN (P101S) mutant strain harboring the PrpoB-ftgA construct. d The ΔcheAW strain. Scale bars, 20 μm.