Fig. 1: Relationship between RAX expression in hypothalamic-pituitary organoids and ACTH secretion.
From: A deep learning approach to predict differentiation outcomes in hypothalamic-pituitary organoids
a Schema of hypothalamic-pituitary interactions in vivo and in vitro. CK, cytokeratin. b Well-formed aggregates and poorly-formed aggregates. The RAX::VENUS cell line was used to confirm the quality of the aggregates. Scale bar = 500 μm. c Immunostaining of hESC aggregates at day 100 for ACTH (white) and LHX3 (red), markers of pituitary differentiation. For RAX::VENUS, spontaneous luminescence of the VENUS protein was imaged without staining. Scale bar = 100 μm. d Culture protocol for hypothalamic-pituitary organoid induction. Aggregates not expressing RAX at day 30 will not secrete ACTH after prolonged cultivation. e Aggregates with large and small RAX expression area groups show differences in ACTH secretion at day 100. Values shown on the graphs represent the means ± SEM, n = 9 independent experiments. *p < 0.05.
