Fig. 1: Systemic Slc25a1 deletion impairs mouse embryonic growth and causes perinatal lethality. | Communications Biology

Fig. 1: Systemic Slc25a1 deletion impairs mouse embryonic growth and causes perinatal lethality.

From: The mitochondrial citrate carrier SLC25A1 regulates metabolic reprogramming and morphogenesis in the developing heart

Fig. 1

A Schematic of the Slc25a1 knockout (KO) first allele. A lacZ-neo KO cassette was inserted between exons 1 and 2 of Slc25a1. B Representative E18.5 Slc25a1+/+, Slc25a1+/-, and Slc25a1-/- embryos obtained from timed intercross of Slc25a1+/- mice. C Quantification of crown-rump lengths of E18.5 embryos of the indicated genotypes (n = 13–26/group). Values reported as mean ± SEM. One-tailed Student’s t test was used for statistical analysis. ****P < 0.0001. D Western blot of SLC25A1 expression in total protein isolated from E17.5 hearts and brains from the indicated genotypes. GAPDH or TGX protein staining was used as loading controls. E Representative immunofluorescence images of SLC25A1 expression in E14.5 hearts from the indicated genotypes. F Representative image of P0 pups obtained from timed intercross of Slc25a1+/- mice. G Genotype frequencies of E18.5 embryos and P0 pups obtained from timed intercross of Slc25a1+/- mice. Illustrations from Clkr.com.

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