Fig. 5: Targeted metabolomic profiling reveals amino acid dysregulation and impaired Krebs cycle function with SLC25A1 deletion. | Communications Biology

Fig. 5: Targeted metabolomic profiling reveals amino acid dysregulation and impaired Krebs cycle function with SLC25A1 deletion.

From: The mitochondrial citrate carrier SLC25A1 regulates metabolic reprogramming and morphogenesis in the developing heart

Fig. 5

A Schematic of the metabolites profiled by GC-MS. B Principal component analysis (PCA) and (C) unbiased hierarchical clustering of profiled analytes showing clear segregation of Slc25a1+/+(n = 6), Slc25a1+/- (n = 3), and Slc25a1-/- (n = 4) hearts. D Normalized abundance of selected metabolites that are significantly altered in HET and KO hearts. Values presented as means ± SEM. One-tailed Student’s t test was used for statistical analysis. P < 0.05 was considered significant, *P < 0.05 and **P < 0.005. E Malate-to-citrate ratios and (F) Fumarate-to-malate ratios in Slc25a1+/+, Slc25a1+/- and Slc25a1-/- hearts. Values presented as means ± SEM. One-way ANOVA followed by a post hoc Dunnett’s test was used for statistical analysis. P < 0.05 was considered significant, ns denotes not significant, *P < 0.05.

Back to article page