Fig. 7: GPR39 small molecule antagonist Z1780628919 as a therapeutic agent for Ang II-induced endothelial cell injury.
From: Targeting GPR39 in structure-based drug discovery reduces Ang II-induced hypertension
a Chemical structure of Z1780628919. b, c Two-dimensional and three-dimensional illustrations of the binding interaction between GPR39 and Z1780628919. GPR39 is shown as a green cartoon, and Z1780628919 as a rose-red stick. 2D and 3D representations were generated using Schrödinger Maestro 11.4 and PyMOL software, respectively. d, e Cartoon and surface diagrams depicting the interaction mode of GPR39 and Z1780628919. GPR39 is illustrated as a green cartoon, and Z1780628919 as a rose-red stick. Images produced with PyMOL software. f–j Analysis of apoptosis marker proteins (P-eNOS, eNOS, Bax, Bcl-2, Caspase-3, and Cleaved caspase-3, n = 6) in HUVECs treated with PBS, Ang II, Z1780628919, and Ang II + Z1780628919. GAPDH was used as a loading control. k–m mRNA expression levels of inflammatory markers (IL-1, IL-6, and TNF-α, n = 6) in HUVECs. Data are expressed as mean ± SEM. Statistical significance is indicated as NS (not significant), *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
