Fig. 7: FOXP2 exerted a protective role in H/R-treated trophoblasts through transcriptionally upregulating LAMA4. | Communications Biology

Fig. 7: FOXP2 exerted a protective role in H/R-treated trophoblasts through transcriptionally upregulating LAMA4.

From: FOXP2 overexpression upregulates LAMA4 expression and thereby alleviates preeclampsia by regulating trophoblast behavior

Fig. 7

A qRT-PCR analysis of the mRNA level of LAMA4. B Western blot analysis of the protein level of LAMA4. C Schematic diagram of LAMA4 promoter-luciferase constructs (left) and luciferase activity was determined by dual luciferase reporter assay in trophoblasts co-transfected with promoter constructs and ovFOXP2/Ev (right). D ChIP-PCR analysis for determining the FOXP2 binding to LAMA4 promoter. E Western blot analysis of the protein level of LAMA4. F Images of migrated and invaded trophoblasts. Scale bar = 100 μm. G The number of migrated and invaded trophoblasts. Data: mean ± SD. N = 3. *p value < 0.05, **p value < 0.01.

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