Fig. 3: Blp bacteriocins-mediated turnover contributes to Ply release. | Communications Biology

Fig. 3: Blp bacteriocins-mediated turnover contributes to Ply release.

From: Pneumococcal pneumonia is driven by increased bacterial turnover due to bacteriocin-mediated intra-strain competition

Fig. 3

A, B Blp bacteriocins mediate Spn turnover in vitro. Overlay inhibition assay depicting lysis of ΔblpMNO overlay strain by producer strains (WT or ΔblpMNO). A Images depicting zones of inhibition in the overlay strain. B Sizes of zones of inhibition. Each dot represents an independent measurement (Arithmetic mean ± standard deviation). P values were calculated using two-tailed t test. C, D Spn turnover by Blp bacteriocins facilitates Ply release. Equal numbers of the two strains were mixed, followed by incubation for 2 h. Equal volumes of the cell-free supernatants from in vitro competition between the strains was loaded and used to detect Ply using (C) anti-Ply immunoblot (rPly was used as a positive control), and D hemolysis of horse erythrocytes (two representative replicates shown, n = 3).

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