Fig. 2: LINC02139 facilitates the malignant phenotypes of GC cells in vitro and in vivo. | Communications Biology

Fig. 2: LINC02139 facilitates the malignant phenotypes of GC cells in vitro and in vivo.

From: LINC02139 interacts with and stabilizes XIAP to regulate cell proliferation and apoptosis in gastric cancer

Fig. 2

A EdU assays were executed to detect the effects of LINC02139 on the DNA synthesis of GC cells. B Colony formation assays were exploited to determine the effects of LINC02139 on the proliferative abilities of GC cells. C, D Transwell assays were employed to examine the migratory (C) and invasive (D) capacities of GC cells after being transfected with vector plasmids, LINC02139 overexpression plasmids, Scr-siRNA, or LINC02139-siRNAp. Scr-siRNA: Scramble-siRNA; siRNAp: siRNA pool, siRNA1, siRNA2, siRNA3. E Wound healing assays were utilized to observe the motility of the transfected GC cells. F The images of the whole body and subcutaneous tumor of the nude mice. G The growth curves of the subcutaneous tumors. ****P < 0.001. H, I Ki-67 and CD105 expression levels in the subcutaneous tumors were tested using IHC assays. Scale bars, 50 μm in (A), 100 μm in (H) and (I).

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