Fig. 3: LINC02139 binds to the XIAP protein in GC cells. | Communications Biology

Fig. 3: LINC02139 binds to the XIAP protein in GC cells.

From: LINC02139 interacts with and stabilizes XIAP to regulate cell proliferation and apoptosis in gastric cancer

Fig. 3

A Proteins retrieved from the LINC02139 pull-down assay in AGS cells were investigated by SDS-PAGE, followed by silver staining. The specific band is denoted with an arrow. B The in vitro transcribed biotinylated full-length of LINC02139 and the antisense control were employed in RNA pull-down assays. The combination of LINC02139 and XIAP was evaluated through western blotting. C RIP assays were conducted with an anti-XIAP antibody in AGS and HGC-27 cells. The enrichment of LINC02139 was measured via qRT-PCR. Anti-IgG was used as a negative control. ****P < 0.001. D Immunoblot detection of the XIAP protein in AGS cells as retrieved by in vitro transcribed biotinylated different constructions of LINC02139. E 6×His-tagged XIAP full-length and truncations were constructed. RIP-qRT-PCR assays were performed to verify the enrichment of LINC02139 in AGS cells by using an anti-His antibody. Anti-IgG was used as a negative control. *P > 0.05, ***P < 0.01.

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