Fig. 4: LINC02139 enhances the stability of XIAP protein. | Communications Biology

Fig. 4: LINC02139 enhances the stability of XIAP protein.

From: LINC02139 interacts with and stabilizes XIAP to regulate cell proliferation and apoptosis in gastric cancer

Fig. 4

A Western blotting analyses of XIAP expression in LINC02139-overexpressed and LINC02139-silenced GC cells. B qRT-PCR analyses of XIAP expression in LINC02139-overexpressed and LINC02139-silenced GC cells. *P > 0.05, ***P < 0.01, ****P < 0.001. C The half-life of XIAP protein was tested after the transfected AGS cells were treated with 100 μM CHX for the indicated time by using western blotting. These pictures were the representatives of three independent experiments with identical results. D The ubiquitin level of XIAP in GC cells was measured using immunoprecipitation and western blotting. IP immunoprecipitation, IB immunoblotting. E The AGS cells were co-incubated with CHX (100 μM) and MG 132 (10 nM) for the indicated periods, followed by western blotting.

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