Fig. 3: Abolishing formation of octasomes by subtracting individual histone. | Communications Biology

Fig. 3: Abolishing formation of octasomes by subtracting individual histone.

From: In vivo assembly of complete eukaryotic nucleosomes and (H3-H4)-only non-canonical nucleosomal particles in the model bacterium Escherichia coli

Fig. 3

a Strain constructs containing different histone components. The symbols ‘+’ and ‘−’ denote the presence and absence of corresponding histone gene, whereas ‘+m’ indicates the histone gene is fused with a mCherry gene. b DNA fragmentation profiles of six strains Ec-AB*34, Ec-pET29a/21a, Ec-B3*4, Ec-A3*4, Ec-AB4*, and Ec-AB3* using ecMNase assay. The green arrow marks the (H3-H4)2 tetrasome, while the red arrow marks mononucleosome particle. M, DNA standards. The complete gel pieces are provided in Supplementary Fig. 6. c Confocal images of five strains Ec-AB*34, Ec-pET29a/21a, Ec-B3*4, Ec-A3*4, Ec-AB4*, and Ec-AB3* using a Leica TCS SP8 STED 3× confocal laser scanning microscope. DAPI was used to stain E. coli DNA, and the ‘fire’ pseudocolor mode of ImageJ was applied to the DAPI images. BF bright field. DAPI mCherry and BF images are shown for the same field of cells. Scale bars = 2 μm.

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