Fig. 4: In vivo assembly of (H3-H4)-only tetrasomes and octasomes. | Communications Biology

Fig. 4: In vivo assembly of (H3-H4)-only tetrasomes and octasomes.

From: In vivo assembly of complete eukaryotic nucleosomes and (H3-H4)-only non-canonical nucleosomal particles in the model bacterium Escherichia coli

Fig. 4

a Strain constructs containing different histone components. The symbols ‘+’ and ‘−’ denote the presence and absence of corresponding histone gene, whereas ‘+m’ indicates the histone gene is fused with a mCherry gene. b DNA fragmentation profiles of five strains: Ec-pET29a/21a, Ec-AB*34, Ec-3*4, Ec-3*, Ec-4* using ecMNase assay. The green arrow marks the (H3-H4)2 tetrasome or (H3-H4)4 octasome, while the red arrow marks mononucleosome particle. M, DNA standards. The complete gel pieces are provided in Supplementary Fig. 6. c Confocal images of three strains Ec-3*4, Ec-3*, and Ec-4* using a Leica TCS SP8 STED 3× confocal laser scanning microscope. DAPI was used to stain E. coli DNA, and the ‘fire’ pseudocolor mode of ImageJ was applied to the DAPI images. BF, bright field. DAPI, mCherry and BF images are shown for the same field of cells. Scale bars = 2 μm.

Back to article page