Fig. 5: In vivo assembly of complete eukaryotic nucleosome and its DNA fragmentation profiles. | Communications Biology

Fig. 5: In vivo assembly of complete eukaryotic nucleosome and its DNA fragmentation profiles.

From: In vivo assembly of complete eukaryotic nucleosomes and (H3-H4)-only non-canonical nucleosomal particles in the model bacterium Escherichia coli

Fig. 5

a A schematic of polycistronic construct, pET21a-H1, for expression of xenopus histone H1 (B4) in E. coli. b Protein expression profiles in strains Ec-pET29a/21a and Ec-AB341. tag-H1, 31.00 kDa; tag-H2A, 18.15 kDa; H2B, 13.64 kDa; H3, 15.07 kDa; tag-H4, 13.31 kDa. c DNA fragmentation profiles of strains Ec-AB34 and Ec-AB341 using ecMNase assay. Arrows mark mono-, and di- nucleosome bands, respectively. M, DNA standards. The complete gel pieces are provided in Supplementary Fig. 6. d Length distribution of over-digestion ecMNase-seq reads from strains Ec-AB34 and Ec-AB341. Source data are provided as Supplementary Data 1. e Thin-section transmission electron photomicrographs of three strains: Ec-pET29a, Ec-AB34, and Ec-AB341 using a H7650 electron microscope. Scale bars = 500 nm.

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