Fig. 3: σB is required for the formation of YTHDF1/3 and stress granules. | Communications Biology

Fig. 3: σB is required for the formation of YTHDF1/3 and stress granules.

From: Infection with novel duck reovirus induces stress granule and methylation-mediated host translational shutoff in Muscovy ducklings

Fig. 3

ICC shows the colocalization of σB and G3BP1 (a) or YTHDF1 (b) compared to that of EV and λA in duck embryo fibroblast CCL-141 cells. Images are shown at 80 × 10 magnification. Scale bars, 20 μm. c–e GF-WB image showing the coexpression (highlighted by the red frame) of YTHDF1, YTHDF3, CAPRIN1, G3BP1, and σB within the same protein fraction (d) compared to that of EVs (c) and λA (e). The numbers marked are the samples collected in sequence. f, g ICC showed increased expression of CAPRIN1 and G3BP1 in the bursa of Fabricius in Muscovy ducklings infected with NDRV HN10 (f) compared to NC (e) in vivo. Images are shown at 4 × 10 (left) and 40 × 10 (right) magnification. Scale bars, 20 μm. h, i Immunogold-labeled TEM image showing the colocalization of G3BP1 (large dots) and YTHDF1 (small dots) in the bursa of Fabricius in Muscovy ducklings infected with NDRV HN10 (h) compared to those infected with NC (g) in vivo. Scale bars, 200 nm (left) and 10 nm (right). ICC immunocytochemistry, GF gel filtration, WB Western blot, TEM transmission immunoelectron microscopy, EV empty vector, NC negative control.

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