Table 2 Resistance phenotypes and genotypes and virulence genes of Klebsiella pneumoniae and its transconjugants

Strains^a	Species	Carbapenemase genes	Virulence genes	Minimal inhibitory concentration^b (MIC, mg/L)
Strains^a	Species	Carbapenemase genes	Virulence genes	IPM	MEM	ATM	AMK	CZA	ATM/A	TGC	CST	CIP
K22877	K. pneumoniae	bla_NDM-5	rmpA, rmpA2, iutAiucABCD and iroBCDN	≥16	≥16	≤1	≤2	≥256	0.5	≤0.5	≤0.5	≤0.25
K23016	K. pneumoniae	bla_NDM-5	rmpA, rmpA2, iutAiucABCD and iroBCDN	≥16	≥16	≤1	≤2	≥256	0.5	≤0.5	≤0.5	≥4
J53	E. coli	–	–	≤0.25	≤0.25	≤1	≤2	≤0.125	≤0.125	≤0.5	≤0.5	–
J53-K22877-T	E. coli	bla_NDM-5	–	≥16	≥16	≤1	≤2	≥256	0.5	≤0.5	≤0.5	–
KP54	K. pneumoniae	–	–	≤0.25	≤0.25	≥64	≥64	≤0.125	≤0.125	2	≤0.5	–
KP54-K22877-T	K. pneumoniae	bla_NDM-5		≥16	≥16	≥64	≥64	≥256	≤0.125	2	≤0.5	–
K56649	K. pneumoniae	bla_KPC-2, bla_NDM-13	rmpA(promotor truncated), rmpA2 and iutAiucABCD	≥16	≥16	≥64	≥64	≥256	0.5	≤0.5	≤0.5	≥4
K60365	K. pneumoniae	bla_KPC-2, bla_NDM-13	rmpA(promotor truncated), rmpA2 and iutAiucABCD	≥16	≥16	≥64	≥64	≥256	0.5	≤0.5	≤0.5	≥4
KP54- K56649-T1	K. pneumoniae	bla_NDM-13	–	≥16	≥16	≥64	≥64	≥256	0.25	2	≤0.5	–
KP54- K56649-T2	K. pneumoniae	bla_KPC-2, bla_NDM-13	–	≥16	≥16	≥64	≥64	≥256	0.5	2	≤0.5	–
J53- K56649-T	E. coli	bla_NDM-13	–	≥16	≥16	≥64	≥64	≥256	0.25	2	≤0.5	–

^a K. pneumoniae K22877 and K23016 belonged to ST65-KL2 CR-hvKP, and K56649 and K60365 belonged to ST11-KL64 hv-CRKP. J53 and KP54 were the recipients for conjugation. J53-K22877-T, KP54- K56649-T1, KP54- K56649-T2 and J53- K56649-T3 belonged to hvCRKP’ transconjugants.
^b IMP, imipenem; MEM, meropenem; ATM, aztreonam; AMK, amikacin, CZA, ceftazidime/avibactam; ATM/A, aztreonam/avibactam; TGC, tigecycline; CST, colistin; CIP, ciprofloxacin. The bold values indicate the MICs mean resistance.

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