Fig. 1: Schematic diagram of this study showing the procedure for extraction and analysis of micronucleus. | Communications Biology

Fig. 1: Schematic diagram of this study showing the procedure for extraction and analysis of micronucleus.

From: Extracting and analyzing micronuclei from mouse two-cell embryos fertilized with freeze-dried spermatozoa

Fig. 1

a mRNA was injected into one-cell stage zygotes, and ACS embryos were classified by fluorescence observation at the two-cell stage. ES cells were also established from the classified ACS embryos. b The relationship between micronuclei and tubulin was observed by live-cell imaging. c The centromeres of micronuclei were observed by FISH. d A method was developed to extract micronuclei from two-cell stage embryos, and NGS analysis of the extracted micronuclei was performed by DNA-seq.

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