Fig. 3: Tubulin dynamics analysis using live cell imaging and detection of major satellite regions in micronuclei. | Communications Biology

Fig. 3: Tubulin dynamics analysis using live cell imaging and detection of major satellite regions in micronuclei.

From: Extracting and analyzing micronuclei from mouse two-cell embryos fertilized with freeze-dried spermatozoa

Fig. 3

a–c Tubulin dynamics were observed from the one-cell to the four-cell stage by live cell imaging. Histones are stained by H2B-mCherry, and tubulin is stained by α-tubulin-EGFP. a NCS embryo. b ACS embryo. Tubulin was still observed after 19 h (b-3). b-4 shows a high magnification of the b-3 square. c ACS embryo. Tubulin disappeared within 6 h. c-4 shows a high magnification of the c-3 square. d, e Major satellite regions of micronuclei at two-cell embryos were observed by DNA-FISH. DNA is stained by DAPI (blue), and the major satellite is stained by DNA-FISH (green). d-3 or e-3 shows high magnification of the d-2 or d-3 square, respectively. The arrow shows a micronucleus with or without a major satellite. Whole Posi: The portion of micronuclei stained by DAPI and the portion stained by DNA-FISH almost overlap. Partial Posi: Only a portion of the DAPI-stained part of the micronucleus is stained by DNA-FISH. Bar, 10 µm.

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