Fig. 2: Characterization of iPSCs-derived lung organoids. | Communications Biology

Fig. 2: Characterization of iPSCs-derived lung organoids.

From: Optimized gene transduction in human lung organoids: A high-efficiency method for advanced research applications

Fig. 2

a–d Quantitative reverse transcription PCR (qRT-PCR) analysis comparing gene expression in iPSCs and iPSC-derived lung organoids across n = 3–11 biologically independent experiments. SFTPB: Surfactant Protein B; SFTPC: Surfactant Protein C; SFTPD: Surfactant Protein D. Data are presented as mean ± SEM, with statistical significance indicated (SFTPB: p = 0.0006; SFTPC: p < 0.0001; ABCA3 p = 0.0021; EPCAM p = 0.0319; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 Welch’s t test). e Confocal fluorescence microscopy images illustrating the expression of SFTPC (magenta) and nuclear staining with DAPI (blue) in human iPSC-derived lung organoids. Scale bar: 100 μm.

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