Fig. 6: Comparison of physical and enzymatic dissociation in human iPSCs-derived lung organoids.

a Brightfield images displaying the morphological changes in human iPSC-derived lung organoids from day 0 to day 7 following physical or enzymatic dissociation. Scale bar: 100 μm. b Brightfield and fluorescence images showing eGFP expression in human iPSC-derived lung organoids 72 h post-transduction under different spinoculation speeds. Scale bar: 300 μm. c Quantification of mean fluorescence intensity of eGFP under various transduction conditions as depicted in (b). Data are presented as mean ± SEM, with statistical significance indicated (static 1.0 h: p = 0.00248, 600g 0.5 h: p = 0.00012, 600g 1.0 h: p = 0.00001, 1200g 0.5 h: p = 0.00006, 1200g 1.0 h: p = 0.0021; *p < 0.05 vs control, Student’s t test). n = 3–5 biologically independent experiments. d Cytotoxicity in fold change of naïve and hACE2-LVV-transduced human iPSC-derived lung organoids in 72 h post-transduction under different spinoculation speeds. Data are presented as mean ± SEM. n = 4 biologically independent experiment.