Table 1 Comparison between previous and current lung organoid transduction methods

Organoid origin

Human iPSCs derived^20,22

Human embryonic and fetal lung derived^3,4,5

Human iPSCs derived

Organoid dissociation

Enzymatic digestion into single cells^{20,22,33,34,35}

Physical breaking into smaller clumps

Cell transduction state

Single cells in iPSC stage^20,22

Single cells in Organoid stage^33,34,35

Small clumps in Organoid stage

Transduction Procedures

Static transduction^{20,22,33,34,35}

Spinoculation

Clone selection/Cell Sorting

Yes^20,34,35

No^22,33

No

Organoid reformation

Required^{20,22,33,34,35}

Not required

Post-transduction recovery on matrigel

yes^{20,22,33,34,35}

No

scRNA sequencing validation

No^20,33,35

Yes^22,34

Yes