Fig. 2: Identification of Ucp1 active enhancers. | Communications Biology

Fig. 2: Identification of Ucp1 active enhancers.

From: Identification of a distal enhancer of Ucp1 essential for thermogenesis and mitochondrial function in brown fat

Fig. 2

a Screenshot from Integrative Genomics Viewer (IGV) showing putative active enhancers of Ucp1. Upper tracks show circularized chromosome conformation capture coupled with next-generation sequencing (4C-seq) chromatin interaction profiles of Ucp1 in iBAT or eWAT. The blue-dashed line represents Ucp1 promoter as a viewpoint. 4C signal is merged across two biological replicates. Lower tracks show ChIP-seq (including H3K27ac, H3K4me1, and RNA Pol II) and ATAC-seq profiles at Ucp1 loci in iBAT and eWAT. Putative active enhancers are indicated by orange rectangles. b Luciferase reporter assay assessing putative active enhancers of Ucp1 in brown adipocytes. On day 8 of differentiation, brown adipocytes were treated with 10 μM isoproterenol (ISO) or vehicle for 4 h before luciferase reporter assay (n = 6). Data are presented as mean ± SEM. Analysis of variance were performed followed by Tukey’s test. c Signal values for chromatin interaction intensity at active enhancer regions in iBAT and eWAT were obtained from bigwig files on IGV. d Signal values for H3K27ac histone enrichment at active enhancer regions in iBAT and eWAT were obtained from bigwig files on IGV.

Back to article page