Fig. 1: hsa_circ_0001599 can promote the odontogenic differentiation of hDPSCs. | Communications Biology

Fig. 1: hsa_circ_0001599 can promote the odontogenic differentiation of hDPSCs.

From: hsa_circ_0001599 promotes odontogenic differentiation of human dental pulp stem cells by increasing ITGA2 expression and stability

Fig. 1

a The expression of hsa_circ_0001599 in hDPSCs. b The expression of hsa_circ_0001599 in dental pulp tissue. c Overexpression efficiency of hsa_circ_0001599 in hDPSCs. d, e The expression level of DSPP, DMP1, RUNX2 and ALP at 7 days and 14 days after odontogenic differentiation using qRT‒PCR. f Following odontogenic differentiation for 7 and 14 days, the expression level of genes related to mineralization can be detected by Western blot analysis. g ALP and ARS staining. h Immunocytochemistry analysis of RUNX2 and ALP expression levels in hDPSCs. a–g All data were presented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, a n = 5 for each group, b n = 4 for each group, c–g n = 3 for each group. g Scale bar, 200 μm, h Scale bar, 50 μm.

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