Fig. 2: The responses of microglia and astrocytes to PNI are heterogeneous. | Communications Biology

Fig. 2: The responses of microglia and astrocytes to PNI are heterogeneous.

From: An atlas of neuropathic pain-associated molecular pathological characteristics in the mouse spinal cord

Fig. 2

a Heatmap shows the top 5 enriched genes in each microglia subcluster. b t-SNE plots depicting the identified microglia subtypes across sham and SNI samples are shown, with colors corresponding to the identified subclusters. In the SNI group, microglia account for 7.57% of the total cells, compared to 5.44% in the sham group. c Histograms displaying the percentage of each microglia subcluster in Sham and SNI groups. After SNI, there was an increase in clusters 1, 2, 3, 4, and 7, while a decrease was observed in clusters 0, 5, 6, and 8, suggesting a significant shift in microglia subpopulations following SNI. d Spatial expression of Classical microglia marker Aif1 and selected subcluster marker genes identified by snRNA-seq, illustrating the heterogeneity of microglia. e The top 5 enriched genes in each astrocyte subcluster. f Astrocyte subtypes in sham and SNI samples are shown in t-SNE plots with corresponding subcluster colors. In the SNI group, astrocytes account for 2.49% of the total cells, compared to 3.61% in the sham group. g SNI caused a shift in astrocyte subpopulations, with an increase in clusters 1, 3, 4, 7, and 9 and a decrease in clusters 0, 2, and 6. h The spatial expression pattern of Gfap and subcluster markers show astrocyte diversity.

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