Fig. 1: scRNA-seq cell clustering.

A Distribution of cells after Harmony batch correction in PC_1 and PC_2, where each point represents a cell; B clustree package displaying clustering at different resolutions; C Visualization of UMAP clustering results depicting the aggregation and distribution of cells from normal samples and GC samples (Normal, N = 3; GC, N = 3), with red representing GC samples and blue representing normal samples; D Visualization of UMAP clustering results illustrating the aggregation and distribution of cells from different source samples, with each color representing a cluster; E Visualization of cell clustering based on UMAP, with each color representing a unique cell subpopulation and unique color borders indicating the distribution range of each subgroup; F Group visualization of cell annotation results based on UMAP clustering; G Expression levels of six cell marker genes in various cell subgroups, where deeper blue indicates higher average expression levels.