Fig. 5: Expression profiles of different TREM2 subtypes and lipid metabolism-related molecules. | Communications Biology

Fig. 5: Expression profiles of different TREM2 subtypes and lipid metabolism-related molecules.

From: Defining gastric cancer ecology: the crucial roles of TREM2+ macrophages and fibroblasts in tumor microenvironments

Fig. 5

A, B mRNA expression levels of TREM2, FABP4, FABP5, and FASN in each subtype of macrophages; C Flow cytometry analysis of the proportion of surface TREM2 in each subtype of macrophages; D Western blot analysis of protein expression levels of TREM2, FABP4, and FABP5 in each subtype of macrophages; E, F Immunofluorescence analysis of TREM2 expression and lipid deposition (blue: nucleus; red: TREM2; green: BODIPY 493/503. Scale bar: 25 μm); G ELISA analysis of IL-10 and Arg1 expression levels in cells; H RT-qPCR analysis of IL-10 and Arg1 expression in cells. Quantitative data are presented as mean ± standard deviation, with intergroup comparisons made using unpaired t-tests. * denotes P < 0.05, ** denotes P < 0.01, *** denotes P < 0.001, and “ns” indicates no statistically significant difference between groups. * denotes statistical significance. Cell experiments were replicated three times.

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