Fig. 7: Effects of the CXCL12/CXCR4 axis expression in CAFs on GC cell proliferation, migration, invasion, and apoptosis. | Communications Biology

Fig. 7: Effects of the CXCL12/CXCR4 axis expression in CAFs on GC cell proliferation, migration, invasion, and apoptosis.

From: Defining gastric cancer ecology: the crucial roles of TREM2+ macrophages and fibroblasts in tumor microenvironments

Fig. 7

A ELISA was performed to detect the concentrations of the cytokines IL-6 and CD206, as well as the chemokine CXCL12 in the cell culture supernatant after M0 differentiation into TAM and MRC-5 into CAF induction; B Typical morphological changes of M0 macrophages co-cultured with CAFs were observed after H&E staining (scale bar: 25 μm); C RT-qPCR was conducted to assess the mRNA expression levels of IL-6 and CD206, markers for M1 and M2 macrophages, respectively; D ELISA was used to measure the concentrations of CXCL12 and CXCR4 in the cell culture supernatant between the CXCL12-neutralized group and the control group; E, F Cell viability and proliferation changes were examined by CCK-8 and colony formation assays; G–I Migration and invasion abilities of the different groups were evaluated by scratch assay and Transwell assay; J Flow cytometry analysis depicted the occurrence of apoptosis in each group, with statistical data on early and late apoptosis presented in the corresponding graphs. Data are expressed as mean ± standard deviation and intergroup comparisons were made using unpaired t-tests. * indicates P < 0.05, ** indicates P < 0.01, and ns denotes no statistical difference between the two groups; * represents the significance level. Cell experiments were repeated three times.

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