Fig. 1: Increased ferroptosis and upregulation of protein arginine methyltransferase 1 (PRMT1), early growth response 1 (EGR1), and glutaminase 2 (GLS2) expression in sepsis-related acute lung injury (ALI) models in vivo. | Communications Biology

Fig. 1: Increased ferroptosis and upregulation of protein arginine methyltransferase 1 (PRMT1), early growth response 1 (EGR1), and glutaminase 2 (GLS2) expression in sepsis-related acute lung injury (ALI) models in vivo.

From: Arginine methyltransferase PRMT1 promotes ferroptosis through EGR1/GLS2 axis in sepsis-related acute lung injury

Fig. 1

The cecal ligation and puncture (CLP) procedure was used to establish a sepsis-induced ALI model. Additionally, ferrostatin-1(Fer-1) was employed to inhibit ferroptosis in sepsis-induced ALI. a Survival curves of mice were analyzed using the Kaplan-Meier method. b Hematoxylin & eosin (HE) staining was performed to examine pathological changes in lung tissue. Scale bar = 100 µm. c Lung injury scores were compared among the Sham group, CLP group, and CLP + Fer-1 group. d Lung weight was measured and the wet-to-dry ratio was calculated in each group. e Changes in mitochondrial morphology were observed using electron microscopy. Scale bar = 500 nm. f–h Levels of malondialdehyde (MDA), 4-hydroxynonenal (4-HNE), and glutathione (GSH) were measured. i The expression levels of PRMT1, EGR1, and GLS2 were detected by Western blot. N = 8 mice, *p < 0.05, **p < 0.01, ***p < 0.001.

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