Fig. 2: Increased ferroptosis and upregulation of protein arginine methyltransferase 1 (PRMT1), early growth response 1 (EGR1) and glutaminase 2 (GLS2) expression in sepsis-related acute lung injury (ALI) models in vitro. | Communications Biology

Fig. 2: Increased ferroptosis and upregulation of protein arginine methyltransferase 1 (PRMT1), early growth response 1 (EGR1) and glutaminase 2 (GLS2) expression in sepsis-related acute lung injury (ALI) models in vitro.

From: Arginine methyltransferase PRMT1 promotes ferroptosis through EGR1/GLS2 axis in sepsis-related acute lung injury

Fig. 2

MLE-12 cells were used to construct an in vitro sepsis-related ALI model by treating them without (control) or with LPS. Afterward, the cells were additionally treated with Fer-1 (LPS+Fer-1). a Cell viability was assessed using the CCK-8 assay. b–e The expression levels of malondialdehyde (MDA), the concentration of Fe2+, glutathione (GSH), and lipid ROS were measured. f The expression levels of PRMT1, EGR1, and GLS2 were detected by Western blot. Mean ± SD, n = 3 independent experiments, and box plots represent median with minimum and maximum whiskers. *p < 0.05, **p < 0.01, ***p < 0.001.

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