Fig. 3: Knockdown of early growth response 1 (EGR1)suppressed ferroptosis in sepsis-related acute lung injury (ALI).
a EGR1 was knocked down in MLE-12 cells by transfecting them with shEGR1 or shNC. The expression levels of EGR1 and GLS2 were detected by Western blot. b–g MLE-12 cells were treated without or with LPS. Afterward, EGR1 was knocked down in those LPS-treated cells by transfecting them with shEGR1 or shNC. b The expression levels of EGR1 and GLS2 were detected by Western blot. c Cell viability was assessed using the CCK-8 assay. d–g The expression levels of malondialdehyde (MDA), the concentration of Fe2+, glutathione (GSH), and lipid ROS were measured. Mean ± SD, n = 3 independent experiments, and box plots represent median with minimum and maximum whiskers. *p < 0.05, **p < 0.01, ***p < 0.001.
