Fig. 5: Protein arginine methyltransferase 1 (PRMT1)-depletion inhibited ferroptosis. | Communications Biology

Fig. 5: Protein arginine methyltransferase 1 (PRMT1)-depletion inhibited ferroptosis.

From: Arginine methyltransferase PRMT1 promotes ferroptosis through EGR1/GLS2 axis in sepsis-related acute lung injury

Fig. 5

a PRMT1 was knocked down in MLE-12 cells by transfecting them with shPRMT1 or shNC. The expression levels of PRMT1, EGR1, and GLS2 were detected by Western blot. b–g MLE-12 cells were treated without or with LPS. Afterward, those LPS-treated cells were transfected with shPRMT1 or shNC. b The expression levels of PRMT1, EGR1, and GLS2 were detected by Western blot. c Cell viability was assessed using the CCK-8 assay. d–g The expression levels of malondialdehyde (MDA), the concentration of Fe2+, glutathione (GSH), and lipid ROS were measured. Mean ± SD, n = 3 independent experiments, and box plots represent median with minimum and maximum whiskers. *p < 0.05, **p < 0.01, ***p < 0.001.

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