Fig. 7: Protein arginine methyltransferase 1 (PRMT1) promoted ferroptosis via early growth response 1 (EGR1)/ glutaminase 2 (GLS2) axis.
a PRMT1 was knocked down and/or EGR1 was overexpressed in MLE-12 cells by transfecting them with shPRMT1 or EGR1 overexpressing vectors or shPRMT1 plus EGR1 overexpressing vectors. The expression levels of PRMT1, EGR1, and GLS2 were detected by Western blot. b–g MLE-12 cells were treated without or with LPS. Afterward, those LPS-treated cells were transfected with shPRMT1 or EGR1 overexpressing vectors or shPRMT1 plus EGR1 overexpressing vectors. b The expression levels of PRMT1, EGR1, and GLS2 were detected by Western blot. c Cell viability was assessed using the CCK-8 assay. d–g The expression levels of malondialdehyde (MDA), the concentration of Fe2+, glutathione (GSH), and lipid ROS were measured. Mean ± SD, n = 3 independent experiments, and box plots represent median with minimum and maximum whiskers. *p < 0.05, **p < 0.01, ***p < 0.001.
