Fig. 8: Knockdown of protein arginine methyltransferase 1 (PRMT1) attenuated sepsis-related ALI by suppressing ferroptosis via early growth response 1 (EGR1)/ glutaminase 2 (GLS2) axis in vivo.

a Survival curves of mice from different groups were analyzed using the Kaplan-Meier method. b HE staining was performed to examine pathological changes in lung tissue. Scale bar = 100 µm. c Lung injury scores were compared among Sham, CLP, CLP + shNC, and CLP+shPRMT1 groups. d Lung weight was measured and the wet-to-dry ratio was calculated in each group. e Changes in mitochondrial morphology were observed using electron microscopy. Scale bar = 500 nm. f–h Levels of malondialdehyde (MDA), 4-hydroxynonenal (4-HNE), and glutathione (GSH) were measured. i, j The expression levels of PRMT1, EGR1, and GLS2 were evaluated by IHC staining and Western blot. Scale bar = 50 µm. N = 8 mice, *p < 0.05, **p < 0.01, ***p < 0.001.