Fig. 2: Assessing pregnant rat model of ferroptosis by intraperitoneal injection of Erastin. | Communications Biology

Fig. 2: Assessing pregnant rat model of ferroptosis by intraperitoneal injection of Erastin.

From: Quercetin alleviates endothelial dysfunction in preeclampsia by inhibiting ferroptosis and inflammation through EGFR binding

Fig. 2

A Schematic illustration of the establishment of an Erastin-induced pregnant rat model. B–B2 The systolic and diastolic pressure from Sham and Erastin groups (B), the systolic blood pressure (B1), and the diastolic blood pressure (B2) on GD17.5 (n = 8 in Sham, n = 9 in Erastin). C The 24-h proteinuria from Sham and Erastin groups (n = 7 in Sham, n = 5 in Erastin). D–F Placental diameter (D) (n = 40 in Sham, n = 63 in Erastin), HE staining of placental sections (E), and placental weight (F) (n = 40 in Sham, n = 63 in Erastin). G–G3 Representative Western blot images of ACSL4, GPX4, and SLC7A11 (G) and bar charts of quantitative analysis of relative expressions of ACSL4 (G1) (n = 18 per group), GPX4 (G2) (n = 12 per group) and SLC7A11 (G3) (n = 18 per group). H–J Detection of MDA concentration (H), GSH concentration (I), and GSH-Px activity (J) in the placenta of rats (n = 5 in Sham, n = 6 in Erastin). K–M The levels of IL-6 (K), IL-10 (L), and TNF-α (M) in the plasma of rats (n = 8 in Sham, n = 6 in Erastin). N The levels of sFlt-1 in plasma of rats (n = 8 in Sham, n = 6 in Erastin). O–P Representative images of Caveolin 1 immunofluorescence staining of labyrinth zone of Sham and Erastin rats (O), and the bar charts of Caveolin 1 (+) vessel density (P) (n = 3 per group). Scale bars = 100 μm in (O). The error bars in (B1–D, F, and H–N) represent the median, and 25–75% quartiles, in (G1–G3, P) represent means ± SD. *P < 0.05; **P < 0.01; ***P < 0.001.

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