Fig. 3: Detection of the cell permeability, ferroptosis, and inflammation. | Communications Biology

Fig. 3: Detection of the cell permeability, ferroptosis, and inflammation.

From: Quercetin alleviates endothelial dysfunction in preeclampsia by inhibiting ferroptosis and inflammation through EGFR binding

Fig. 3

A CCK-8 shows the effect of Erastin on cell viability. B CCK-8 shows the effect of QCT on cell viability. C CCK-8 shows the effect of Erastin 24 h and QCT 48 h on cell viability (n = 3 per group). D CCK-8 shows the effect of QCT 24 h, Erastin, and QCT 24 h on cell viability (n = 3 per group). E, F Representative images of PI staining of HUVEC from the Control, Erastin, and Erastin + QCT groups (E) and the bar charts of the ratio of PI-positive cells (F) (n = 3 per group). G Representative western blot images and bar charts of quantitative analysis of relative expressions of ACSL4 (n = 4 per group), GPX4 (n = 5 per group), and SLC7A11 (n = 3 per group) from the aforementioned three groups. H The relative mRNA expression of IL-6, IL-10, and TNF-α in HUVEC from the aforementioned three groups (n = 3 per group). I–J Representative fluorescent detection of DCFH-DA from the aforementioned three groups (I) and the bar charts of ROS mean fluorescent intensity (J) (n = 3 per group). K–L Representative fluorescent detection of JC-1 from the aforementioned three groups (E) and the bar charts of the ratio of red/green fluorescent intensity (F) (n = 3 per group). Scale bars = 100 μm in (E), (I), and (K). The error bars in the figure represent means ± SD. *P < 0.05; **P < 0.01; ***P < 0.001.

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