Fig. 6: Assessing the cell death, ROS, mitochondrial membrane potential change, and endothelial function and detection of downstream cell signaling pathway protein changes after down-regulation of EGFR. | Communications Biology

Fig. 6: Assessing the cell death, ROS, mitochondrial membrane potential change, and endothelial function and detection of downstream cell signaling pathway protein changes after down-regulation of EGFR.

From: Quercetin alleviates endothelial dysfunction in preeclampsia by inhibiting ferroptosis and inflammation through EGFR binding

Fig. 6

A–F The bar charts of the ratio of PI-positive cells (A), ROS mean fluorescent intensity (B), the ratio of red/green fluorescent intensity (C), ZO-1 mean fluorescent intensity (D), 24 h (E) and 48 h (F) relative wound closure from the si-NC, si-NC + Erastin, si-NC + Erastin + QCT, si-EGFR, siEGFR + Erastin and si-EGFR + Erastin + QCT groups (n = 6 per group). G–G5 Representative western blot images of EGFR, pEGFR, AKT, pAKT, ERK, pERK and GPX4 (G) and bar charts of quantitative analysis of relative expressions of EGFR (G1), pEGFR/EGFR (G2) from the si-NC, si-NC + Erastin and si-NC + Erastin + QCT groups (n = 3 per group), and relative expressions of pAKT/AKT (G3), pERK/ERK (G4) and GPX4 (G5) from the si-NC, si-NC + Erastin, si-NC + Erastin + QCT, si-EGFR, siEGFR + Erastin and si-EGFR + Erastin + QCT groups (n = 3 per group). The error bars in the figure represent means ± SD. ns no significance; *P < 0.05; **P < 0.01; ***P < 0.001; #P < 0.05; ##P < 0.01; ###P < 0.001.

Back to article page