Fig. 8: Detection of the ferroptosis, inflammation, and endothelial function.

A–A3 Representative Western blot images of ACSL4, GPX4, and SLC7A11 (A) and bar charts of quantitative analysis of relative expressions of ACSL4 (A1) (n = 8 per group), GPX4 (A2) (n = 9 per group) and SLC7A11 (A3) (n = 11 per group) from the Sham, sRUPP, sRUPP + Fer-1 and sRUPP + QCT groups. B–D The levels of MDA concentration (B), GSH concentration (C), and GSH-Px activity (D) in the placenta of the aforementioned four groups (n = 7 in Sham, n = 7 in sRUPP, n = 5 in sRUPP + Fer-1, n = 5 in sRUPP + QCT). E–G Detection of IL-6 (E) (n = 7 in Sham, n = 9 in sRUPP, n = 6 in sRUPP + Fer-1, n = 8 in sRUPP + QCT), IL-10 (F) (n = 7 in Sham, n = 10 in sRUPP, n = 6 in sRUPP + Fer-1, n = 8 in sRUPP + QCT), and TNF-α (G) (n = 7 in Sham, n = 9 in sRUPP, n = 6 in sRUPP + Fer-1, n = 8 in sRUPP + QCT) in plasma of the aforementioned four groups. H The levels of sFlt-1 in plasma of the aforementioned four groups (n = 8 in Sham, n = 9 in sRUPP, n = 6 in sRUPP + Fer-1, n = 8 in sRUPP + QCT). I–J Representative images of Caveolin 1 immunofluorescence staining of labyrinth zone of the aforementioned four groups rats (I), and the bar charts of Caveolin 1 (+) vessel density (J) (n = 3 per group). Scale bars = 100 μm in (J). The error bars in (A1–A3, J) represent means ± SD, in (B–H) represent median and 25–75% quartiles. ns no significance; ^*P < 0.05; ^**P < 0.01; ^***P < 0.001.