Fig. 3: MAG3 mediates the stable association of ERESs with Golgi stacks at the ER-Golgi interface. | Communications Biology

Fig. 3: MAG3 mediates the stable association of ERESs with Golgi stacks at the ER-Golgi interface.

From: Plant-specific tail-anchored coiled-coil protein MAG3 stabilizes Golgi-associated ERESs to facilitate protein exit from the ER

Fig. 3

a Representative VAEM images of cotyledon epidermal cells expressing GFP-MAG3 and MAG5-mCherry (ERES marker), showing MAG3 localizes close to Golgi-associated ERES (circled), but not to Golgi-free ERES (arrowheads). b Colocalization analysis showing Pearson correlation coefficient between MAG3 and ERES marker (MAG5-mCherry). Lines indicate the median and boxes indicate 25% and 75% quartiles. Whiskers indicate the minimum and maximum values within 1.5× the interquartile range. ***P < 0.001 (Two-sided Wilcoxon rank-sum test, P < 2.2 × 10−16). n = 114 Golgi-associated ERESs from 36 cells. n = 114 free ERESs from 36 cells. c Time series of representative VAEM images of cotyledon epidermal cells expressing GFP-MAG3 and MAG5-mCherry (ERES marker) for 1.4 s. Note that an ERES dissociates from MAG3 signals when it is released from a Golgi stack (arrowheads). See also Supplementary Fig. 4a for two replicates and Supplementary Video 2 for the original real-time movie. d Representative VAEM images of cotyledon epidermal cells of mag3-5 and the control line (MAG3), both of which expressed MAG5-GFP (ERES marker) and mCherry-SYP32 (cis-Golgi marker). Circles indicate Golgi-associated ERESs. e Statistical analysis of relative intensity of Golgi-associated ERES in cotyledon epidermal cells of mag3-5 and the control line (MAG3) expressing MAG5-GFP and mCherry-SYP32 (cis-Golgi marker). Relative intensity of Golgi-associated ERES was defined as the ratio of GFP fluorescence intensity of ERESs surrounding a single Golgi stack to the average intensity of a single free ERES. Data are represented as mean ± SD (n = 104 from 21 cells of the control plants and n = 116 from 32 cells of mag3-5). ***P < 0.001 (Two-sided Welch’s t test, P = 1.5 × 10−21). f Time series of representative VAEM images of cotyledon epidermal cells of mag3-5 expressing MAG5-GFP (ERES marker) and mCherry-SYP32 (cis-Golgi marker) for 1.4 s. Arrowheads indicate punctate ERESs being captured by a Golgi stack. See also Supplementary Fig. 4b for two replicates and Supplementary Video 3 for the original real-time movie.

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