Fig. 1: PfFBXO1 shows a dynamic localization during schizont development. | Communications Biology

Fig. 1: PfFBXO1 shows a dynamic localization during schizont development.

From: PfFBXO1 is essential for inner membrane complex formation in Plasmodium falciparum during both asexual and transmission stages

Fig. 1

PfFBXO1 localization by immunofluorescence stained with anti-V5 (PfFBXO1) and A anti-CrCen (Centrin, centrosome marker), (B) anti-PfGAP45 (IMC marker), or (C) anti-myc (PfBLEB, basal complex marker). Scale bar = 2 µm for (A–C). Ultrastructure Expansion Microscopy (U-ExM) of early stage schizont showing PfFBXO1 localizing to the developing daughter cell scaffold and co-stained with (D) anti-CrCen (Centrin) or (E) anti-PfGAP45 (IMC). Scale bar = 5 µm for (D, E). Inset panel shows 5X zoomed image. Shown here are representative images from at least 3 biological replicates. F Western blot of smV5-tagged PfFBXO1 showing the expression throughout schizont development, indicated by hours post-infection above each lane. G Graph showing the relative expression of smV5-tagged PfFBXO1 protein at respective time points. Values represented are mean with SEM from two biological replicates, each in done in technical triplicate.

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