Fig. 2: PfFBXO1 localizes to the IMC in gametocytes. | Communications Biology

Fig. 2: PfFBXO1 localizes to the IMC in gametocytes.

From: PfFBXO1 is essential for inner membrane complex formation in Plasmodium falciparum during both asexual and transmission stages

Fig. 2

Localization of PfFBXO1 by immunofluorescence in gametocyte stages stained with anti-V5 (PfFBXO1) and (A) anti-PfGAP45 (IMC marker), (B) anti-alpha-Tubulin (sub-pellicular microtubule, SPMT, marker), or (C). anti-myc (PfBLEB). Shown here are max-projections with Scale bar = 2 µm for (A–C). U-ExM (max-projections) showing PfFBXO1 localization at (D) the IMC plates similar to PfGAP45 in stage II gametocyte, (E) exterior to that of SPMTs visualized by anti-alpha-Tubulin in a stage IV gametocyte, and (F) single z-stack of an unexpanded stage IV gametocyte measuring the distance between two peaks of SPMT staining by anti-alpha-tubulin and anti-V5 (PfFBXO1) staining along the gray line. G Max-projection of U-ExM showing PfFBXO1 distinct from PfBLEB compartment in a stage III gametocyte. Scale bar for U-ExM = 10 µm (D, E and G). Shown here are representative images of results from at least 3 biological replicates.

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