Fig. 6: Live cell microscopy of gametocyte development and maturation shows morphological defects in rapamycin treated PfFBXO1 knockout gametocytes.
A Representative images of control and knockout stage II gametocyte on Day 4, stage III on Day 6, and stage IV on Day 8 post-induction showing increased abnormalities in shape and SPMTs (visualized by SirTubulin) upon rapamycin treatment as gametocytogenesis progresses. Scale bar = 2 µm. B Scatter plot quantifying the shape of the gametocyte depicts increased roundness upon rapamycin treatment in PfFBXOmNG gametocytes compared to controls, as measured in live gametocytes by confocal microscopy. The results plotted in the graph are values from three technical triplicates showing median (line) with 95%CI (bars). Significance is calculated using unpaired two-tailed t-test with Welch’s correction.
