Fig. 8: PfFBXO1 alone is insufficient to complement TgFBXO1. | Communications Biology

Fig. 8: PfFBXO1 alone is insufficient to complement TgFBXO1.

From: PfFBXO1 is essential for inner membrane complex formation in Plasmodium falciparum during both asexual and transmission stages

Fig. 8

A List of complement parasite strains generated with details of FBXO1 protein expressed. Endogenous HATgFBXO1 expression is controlled by ATc. Constitutive expression of myc-tagged FBXO1 complements is controlled by SAG4 promoter. B Western blot depicting the efficacy of HA-tagged endogenous TgFBXO1 knockdown. C Western blot showing the expression of different myc-tagged complements. The top band in each lane is the full-length protein, and lower bands likely represent breakdown products. D Plaque assays showing the effect of complementation with exogenous myc-tagged-FBXO1 in the absence of endogenous HATgFBXO1. Scale bar = 2 mm. E Scatter plot quantifying the plaque areas measured upon complementation shows successful complementation with a chimeric-FBXO1 but not with full-length PfFBXO1 alone. The plaque assays were performed at the same time in biological triplicates and the combined data is plotted (N = 30 for each condition). The significance was calculated using ordinary one-way ANOVA (**** represents p < 0.0001).

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