Fig. 5: Effects of AAVi-Ctnnb1 administration on neuronal cell damage and survivin in CA1 after tGCI with or without hypoxia. | Communications Biology

Fig. 5: Effects of AAVi-Ctnnb1 administration on neuronal cell damage and survivin in CA1 after tGCI with or without hypoxia.

From: Inhibition of SCFKDM2A/USP22-dependent nuclear β-catenin ubiquitylation mediates cerebral ischemic tolerance

Fig. 5

A Phase contrast and fluorescent images from coronal sections of CA1 following injection of AAVi-CON (a, b) or AAVi-Ctnnb1 (c, d) in Sham animals. Black arrowhead shows the needle trace. Scale bar: 75 μm. B Representative immunoblots of β-catenin expression in CA1 after AAVi-Ctnnb1 administration at the dosage of 3.96 × 109 v.g, 7.92 × 109 v.g or 15.84 × 109 v.g in Sham rats. Data are expressed as percentage of value of Sham animals with AAVi-CON. &p < 0.05 vs. Sham group with AAVi-CON (p = 0.001, n = 3, One-Way ANOVA Test, p = 0.871 at 3.96× 109 v.g, p = 0.015 at 7.92× 109 v.g, p = 0.002 at 15.84× 109 v.g, Bonferroni post-test). C Representative microphotographs of cresyl violet staining and NeuN immunostaining in CA1 from rats administered bilaterally with either AAVi-CON or AAVi-Ctnnb1 at 7 d after tGCI with hypoxia or without hypoxia. Boxes indicate that the magnified regions displayed in the right panel. Scale bar: a, c, e, g, i, k, m, o, q, s, u w: 250 μm; b, d, f, h, j, l, n, p, r, t, v, w: 25 μm. D, E Quantitative analyses of surviving and NeuN-positive cells in CA1. *p < 0.05 vs. Sham animals (p < 0.001 for surviving cells, p < 0.001 for NeuN-positive cells, n = 3, unpaired t-test), &p < 0.05 vs. the same group with AAVi-CON (for surviving cells, p = 0.438 in the Sham group with AAVi-Ctnnb1, p = 0.040 in the HPC group with AAVi-Ctnnb1; for NeuN-positive cells, p = 0.714 in the Sham group with AAVi-Ctnnb1, p = 0.046 in the tGCI group with AAVi-Ctnnb1, unpaired t-test). F, G Western blot analyses of nuclear β-catenin and survivin in CA1. The histogram presents the quantitative analyses of nuclear β-catenin and survivin levels. Data are expressed as percentage of value of Sham animals. The nuclear fraction purity was assessed by absence of GAPDH staining in Figure F and G. *p < 0.05 vs. Sham group (p = 0.023 for β-catenin, n = 3; p = 0.013 for survivin, n = 4, unpaired t-test), &p < 0.05 vs. the same group with AAVi-CON (for β-catenin, p = 0.047 at the Sham group with AAVi-Ctnnb1, p = 0.041 in the HPC group with AAVi-Ctnnb1; for survivin, p = 0.016 in the Sham group with AAVi-Ctnnb1, p = 0.007 in the HPC group with AAVi-Ctnnb1, unpaired t-test). Each bar represents the mean ± SD (error bars).

Back to article page