Fig. 5: Co-expression of VapB3 restores the growth defect associated with overexpression of VapC35 in M. smegmatis. | Communications Biology

Fig. 5: Co-expression of VapB3 restores the growth defect associated with overexpression of VapC35 in M. smegmatis.

From: Understanding the physiological role and cross-interaction network of VapBC35 toxin-antitoxin system from Mycobacterium tuberculosis

Fig. 5

A–D M. smegmatis harbouring an Atc inducible integrative and Ac inducible episomal copy of vapC35 and antitoxin (vapB, (A), mazE, (B), relB, (C) and parE, (D)) were grown till early log phase (OD600nm ~ 0.2–0.3). The expression of toxin and antitoxin was induced by the addition of Atc and Ac, respectively. The heat map represents the growth patterns of various M. smegmatis strains as determined by measuring OD600nm at regular intervals. E Growth kinetics of M. smegmatis harbouring an integrative copy of VapC35 either alone or in the presence of VapB35, VapB3, VapB5, VapB19, VapB29, VapB38, VapB46 or VapB47. The growth of various strains was determined by measuring OD600nm at regular intervals. The data shown in (A–E) are representative of two independent experiments. F The induced cultures were serially diluted at time zero, 12 h post-induction, 24 h post-induction and spotted on MB7H11 plates at 37 °C for 48 h. The images shown in this panel are representative of two independent experiments. Source data is provided in supplementary data 1.

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