Table 1 The mass fractions and corresponding molecular weights of each peak for the proteins analysed are represented (T: Toxin, A: Antitoxin)
Proteins | Peaks | Observed molecular weight (KDa) | Expected molecular weight (KDa) | Mass fraction | Stoichiometry |
|---|---|---|---|---|---|
VapB35 | Peak1 | 22.9 | 22.8 | 97.2 | A2 (Dimer) |
VapC35 | Peak1 | 25.2 | 32.2 | 96.7 | T2 (Dimer) |
VapB35:VapC35 (1:4) | Peak1 Peak2 | 55.7 91.2 | 32.2/22.8 87.6 | 19.5 80.5 | T2/A2 (Dimer) T2A2T2 (Hetero-hexamer) |
VapB35:VapC35 (1:2) | Peak1 Peak2 | 50.4 92.0 | 32.2/22.8 87.6 | 63.5 36.5 | T2/A2 (Dimer) T2A2T2 (Hetero-hexamer) |
VapB35:VapC35 (1:1) | Peak1 | 113.8 | 110.0 | 97.5 | T4A4 (Hetero-octamer) |
VapB35:VapC35 (2:1) | Peak1 | 104.3 | 110.0 | 90.2 | T4A4 (Hetero-octamer) |
VapB35:VapC35 (4:1) | Peak 1 | 123.4 | 110.0 | 86.2 | T4A4 (Hetero-octamer) |
VapB3 | Peak1 | 31.0 | 30.2 | 99.9 | A2 (Dimer) |
VapC3 | Peak1 Peak2 | 42.8 10310 | 40.2 n.a. | 23.4 76.6 | T2 (Dimer) Aggregate |
VapB3:VapC3 (1:2) | Peak1 Peak2 Peak3 | 166.8 267.2 4026 | 191.0 281.6 n.a. | 72.7 21.6 5.7 | T2A2T2 (Hetero-hexamer) T4A4 (Hetero-octamer) Aggregate |
VapB3:VapC3 (1:1) | Peak1 Peak2 | 159.4 270.4 | 191.0 281.6 | 75.7 24.3 | T2A2T2 (Hetero-hexamer) T4A4 (Hetero-octamer) |
VapB3:VapC3 (2:1) | Peak1 Peak2 Peak3 | 34.6 161.4 260.5 | 30.2/40.2 191.0 281.6 | 41.9 48.3 9.8 | T2/A2 (Dimer) T2A2T2 (Hetero-hexamer) T4A4 (Hetero-octamer) |
VapB3:VapC35 (1:1) | Peak1 Peak2 Peak3 | 141.2 178.5 3187 | 159.0 189.2 n.a. | 73.2 25.2 1.6 | T2A2T2 (Hetero-hexamer) T4A4 (Hetero-octamer) Aggregate |
VapB3:VapC35 (2:1) | Peak1 Peak2 Peak3 | 136.4 207.0 4230 | 159.0 189.2 n.a. | 77.0 13.6 9.4 | T2A2T2 (Hetero-hexamer) T4A4 (Hetero-octamer) Aggregate |
