Fig. 8: Weighted gene co-expression network analysis (WGCNA) of metabolites and expressed genes. | Communications Biology

Fig. 8: Weighted gene co-expression network analysis (WGCNA) of metabolites and expressed genes.

From: Integrated multi-omics analysis unravels the floral scent characteristics and regulation in “Hutou” multi-petal jasmine

Fig. 8

a Hierarchical cluster dendrogram. Each branch represents a module, and each leaf corresponds to an individual gene. Different colors represent different modules. b Correlation heatmap between the 16 floral SDVs and 11 gene modules. The x-axis presents each volatile index (trait), while the y-axis presents each module. The colored cells represent the correlation coefficient between the module and SDV content, with darker shades of red reflecting a stronger positive correlation. c Expression heatmaps and consensus profiles of co-expressed genes in the MEmagenta module. The upper section displays a clustered heatmap of genes within the module (red and green represent high and low expression, respectively), while the lower section represents the expression pattern of the module eigengene across different samples. d Hub gene network (top 150 genes) in the MEmagenta module visualized by Cytoscape (v 3.10.2). The red solid points indicate the key hub genes. e Phylogenetic tree of HTWRKY41, HTWRKY53 and AtWRKY family (Arabidopsis thaliana) proteins. f Multiple alignment of HTWRKY41 and HTWRKY53 proteins with AtWRKY41 and AtWRKY53 was performed using DNAMAN (v 10). The sequence in red represents the conserved heptapeptide WRKYGQK domain, and the sequence in blue represents the C2-HC (C-X7-C-X23-H-X-C)-type zinc finger protein domain. Black, pink, and blue colors indicate 100%, 75%, and 50% sequence consistency, respectively. g Subcellular localization of the HTWRKY53 protein in tobacco protoplast. GFP: green fluorescent protein; mKATE: nuclear marker; Cy5: chloroplast; Bright: bright field; Merged: combined mKATE and GFP and bright field; Bars, 10 μm. h Transcriptional capacity of hub transcription factor HTWRKY53 in tobacco leaves. Data are presented as mean ± standard deviations (SD) from three biological replicates. BD-62SK vector and BD-62SK-VP16 are used as negative and positive controls, respectively. Significant differences compared to the empty BD-62SK vector are marked with asterisks above the bars (**P-value < 0.01).

Back to article page