Fig. 2: Identification of MMT in CaOx nephrocalcinosis mice. | Communications Biology

Fig. 2: Identification of MMT in CaOx nephrocalcinosis mice.

From: EZH2-mediated macrophage-to-myofibroblast transition contributes to calcium oxalate crystal-induced kidney fibrosis

Fig. 2

A Schematic diagram of the experimental procedure, Created in BioRender. Yuqi, X. (2025) https://BioRender.com/z03c224. B The deposited crystals were identified to be CaOx by infrared spectroscopy. C Renal function, body weight and hydroxyproline content were measured on day 12 of modeling (n = 6). D Representative pictures of HE, Von Kossal, and Masson staining (magnification, ×400). E Quantifying kidney tubular injury score, CaOx crystal deposition area, and collagen deposition area (n = 6). F Transcriptomic sequencing in kidney tissues between CaOx nephrocalcinosis mice and the control group (n = 3). G Two-color immunofluorescence identifies MMT cells that coexpress macrophage (CD68, green) and myofibroblast (a-SMA, red) markers in nephrocalcinosis mice. H Quantification of the number of CD68+ cells, a-SMA+ cells, and CD68+ a-SMA+ cells (n = 6). Data are presented as the mean ± SEM. *P < 0.05 versus control group. P values were determined by the two-tailed Student’s t test.

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