Fig. 4: EZH2 is markedly elevated in CaOx crystal-induced kidney fibrosis.

A GO enrichment analysis of differentially expressed genes between normal and nephrocalcinosis mice. B Heatmap of histone methylation enzyme-related gene expression (n = 3). C Representative pictures of EZH2 immunohistochemistry staining in patients with nephrocalcinosis-related CKD. D The gene activity of Ezh2 in different renal cell types from AKI mouse kidney (from snATAC-Seq database⁴⁹; http://humphreyslab.com/SingleCell/). E Two-color immunofluorescence identifies EZH2 (red) was increased in F4/80⁺ (green) macrophages during nephrocalcinosis. F Quantifying the number of F4/80⁺ and F4/80⁺ EZH2⁺ cells (n = 6). Expression of EZH2 was detected by immunoblotting in nephrocalcinosis mice (G, H) and calcium oxalate monohydrate (COM)-treated macrophages (I, J) (n = 3). Data are presented as the mean ± SEM. *p < 0.05 versus control group. P values were determined by the two-tailed Student’s t test.